Conserved Locus-Specific Silencing Functions of Schizosaccharomyces pombe sir2+

Conserved locus-specific silencing functions of Schizosaccharomyces pombe sir2+.

In Schizosaccharomyces pombe, three genes, sir2(+), hst2(+), and hst4(+), encode members of the Sir2 family of conserved NAD(+)-dependent protein deacetylases. The S. pombe sir2(+) gene encodes a nuclear protein that is not essential for viability or for resistance to treatment with UV or a microtubule-destabilizing agent. However, sir2(+) is essential for full transcriptional silencing of cent...

New alleles of SIR2 define cell-cycle-specific silencing functions.

The establishment of transcriptional silencing in yeast requires cell-cycle progression, but the nature of this requirement is unknown. Sir2 is a protein deacetylase that is required for gene silencing in yeast. We have used temperature-sensitive alleles of the SIR2 gene to assess Sir2's contribution to silencing as a function of the cell cycle. When examined in vivo, these conditional alleles ...

Allelic Complementation at the Adenylosuccinase Locus in Schizosaccharomyces Pombe.

OMPLEMENTATION between allelic mutants at the ad-4 (adenine-4) locus, deficient for the enzyme adenylosuccinase (adenylosuccinate AMP-lyase, C.E. 4322). was first reported in Neurospora (GILES, PARTRIDGE and NELSON 1957; WOODWARD, PARTRIDGE and GILES 1958). It was found that certain pairs of these mutants, which as a result of the absence of adenylosuccinase activity require adenine for growth,...

[Schizosaccharomyces pombe].

A Novel Epigenetic Silencing Pathway Involving the Highly Conserved 5’-3’ Exoribonuclease Dhp1/Rat1/Xrn2 in Schizosaccharomyces pombe

Epigenetic gene silencing plays a critical role in regulating gene expression and contributes to organismal development and cell fate acquisition in eukaryotes. In fission yeast, Schizosaccharomyces pombe, heterochromatin-associated gene silencing is known to be mediated by RNA processing pathways including RNA interference (RNAi) and a 3'-5' exoribonuclease complex, the exosome. Here, we repor...